VGLUTs in Peripheral Neurons and the Spinal Cord: Time for a Review
Figure 2
Frequent coexpression of VGLUT 2 or EGFP (VGLUT 3) with TH or VGLUT 2 and EGFP in mouse nonvisceral DRG neurons. Immunofluorescence photomicrographs of nonvisceral DRG sections incubated with VGLUT1 ((a), (d)), VGLUT2 ((b), (e)), or EGFP antibodies ((c), (d), (e)). ((a)–(c)) While virtually no VGLUT1-IR (arrowheads in (a)) DRG NPs coexpress with TH (arrows in (a)), most VGLUT2- or EGFP-IR NPs present with TH-Li (black double arrowheads in (b), (c)). Abundant VGLUT2- (arrowheads in (b)), EGFP- (arrowheads in (c)), and some TH-only (arrows in (c)) expressing NPs are also detected. ((d), (e)) Lack of coexpression is observed between VGLUT1 (arrowheads in (d)) and EGFP (arrows in (d)). In contrast, EGFP is virtually always coexpressed with VGLUT2 in DRG NPs (double black arrowheads in (e)). Many other VGLUT2-only expressing DRG NPs are also detected (arrowheads in (e)). Scale bars: 30 μm ((c) = (a), (b); (e) = (d)). Data in figures (a)–(e) is previously unpublished, and the tissue has been processed according to the immunohistochemical procedures described in [54, 60–62].
