UV-C Exposure Induces an Apoptosis-Like Process in Euglena gracilis
Figure 4
Agarose gel electrophoresis. Markers for (a) and (c) are 1 kb ladder and pBR322 BstN1 cut for (b). (a) Cells exposed to UV for different times and allowed to sit in dark for 18 hours before DNA isolation. From left to right: control, 1 min, 3 min, 5 min, 10 min, 20 min, and 30 min. (b) Cells exposed to UV light for 30-minutes and DNA was isolated after sitting in dark for different time periods. From left to right: control, 3 hr, 6 hr, 9 hr, 12 hr, 15 hr, and 20 hr. (c) From left to right: (1) control; (2) 30-minute UV exposure and DNA isolated 18 hours later after sitting in dark; (3) preincubation with 20 μM Z-VAD-FMK, then 30 minute UV exposure and DNA isolated 18 hours later after sitting in dark; (4) treated with 1 μM staurosporine and DNA isolated 18 hours later.
