Evaluation of the In Vitro Efficacy of Artemisia annua, Rumex abyssinicus, and Catha edulis Forsk Extracts in Cancer and Trypanosoma brucei Cells
Figure 3
Inhibition of cancer cell growth by Rumex abyssinicus (RMA) extract. A colorimetric assay was used to determine proliferation of tumour cells (5000 cells/well in a 96 well plate) exposed to different concentrations of RMA extract (3–333 μg/mL). After incubation periods of 24 hrs, 12 μL stock solution of WST-1 was added to each well plate and incubated for 4 hrs at 37°C. As a control, DMSO was added instead of the plant extract (zero value). The absorbance was measured at a wavelength of 450/620 nm by an ELISA reader. The reduction in cell number was calculated from the absorbance given in percent. Statistical significance was calculated using unpaired -test, at 95% CI (*).
