Locus-Specific Biochemical Epigenetics/Chromatin Biochemistry by Insertional Chromatin Immunoprecipitation

Figure 1

Scheme of ChIP. In ChIP, molecular interaction can be preserved by crosslinking with formaldehyde or other crosslinkers. Subsequently, chromatin is fragmented by sonication or digestion with endonucleases. Immunoprecipitation with antibodies against DNA-binding proteins of interest is performed to isolate genomic regions bound by the DNA-binding proteins.