Locus-Specific Biochemical Epigenetics/Chromatin Biochemistry by Insertional Chromatin Immunoprecipitation

Figure 2

Scheme of 3C-based identification of interacting genomic regions. In 3C, molecular interaction is maintained by crosslinking with formaldehyde before digesting with a restriction enzyme(s). After ligation of DNA ends in the same complex, crosslink is reversed and DNA is purified. Interaction of genomic loci is detected by PCR using locus-specific primers or microarray/NGS.