Locus-Specific Biochemical Epigenetics/Chromatin Biochemistry by Insertional Chromatin Immunoprecipitation
Figure 5
Knock-in and transgenic approaches of iChIP. (a) Knocking-in of LexA BE into the endogenous locus or (b) random integration of transgenes retaining LexA BE can be used for iChIP. Zinc-finger nucleases (ZFN) and TALEN technology make gene targeting much easier in cultured cell lines.