Research Article
Development and Validation of a Stability Indicating RP-UPLC Method for Analysis of Imipramine Hydrochloride in Pharmaceuticals
Table 1
Comparison of the performance characteristics of the proposed method with some of the existing chromatographic/spectrophotometric methods.
| Sl No. | Reagent/s used | Methodology | Linear range | Remarks | References |
| 1 | NaClO4—buffer ethanol | Derivative spectrophotometry | 0.62–10.14 g/mL | Sensitive but lack of selectivity | [19] |
| 2 | Methyl orange | Formation of ion pair with methyl orange and the absorbance was measured at 425 nm | 0.79–25.3 g/mL | Less sensitive | [21] |
| 3 | Eriochrome cyanine R | Reacts in neutral medium with imipramine forming reddish compound | 10–80 g/mL | Less sensitive and has narrow range | [22] |
| 4 | Iminodibenzyl p-chloranilic acid | First derivative of ratio spectra Formation of purple colored charge transfer complex | 5–30 g/mL 20–200 g/mL | Less sensitive and very narrow range | [20] |
| 5 | Azocarmine G Naphthalene blue Woolfast blue BL | Extracts of the ion associates exhibit absorption maxima at 550 (ACG), 620 (NB), and 590 (WFB BL) nm | 2.0–12.0 g/mL 4.0–16.0 g/mL 1.0–12.0 g/mL | Sensitive but requires tedious extraction procedures | [23] |
| 6 | 3-Methylbenzothiazolin-2-one hydrazone-iron(III)chloride | Oxidative coupling of the drugs with 3-methylbenzothiazolin-2-one hydrazone which shows maximum absorption at 630 nm | 1.0–25 g/mL ( = 3.20 × 103) | Less sensitive | [24] |
| 7 | Mobile phase consisting of a mixture of acetonitrile and ammonium acetate buffer of pH-5 (30 : 30 : 40, v/v/v) | UPLC | 0.2–3 g/mL | Highly sensitive and selective | Developed method |
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