Challenges in Enzymatic Route of Mannitol Production

Figure 2

Mannitol production pathways: double arrow (→→) heterofermentative and single arrow homofermentative (→) LAB while sorbitol production pathway shown by broken arrow (→) is. Enzymes are numbered, (1) glucose permease, (2) fructose permease, (3) glucokinase, (4) fructokinase, (5) phosphoglucose isomerase, (6) mannitol-1-phosphate dehydrogenase, (7) PEP dependentPTS, (8) mannitol dehydrogenase, (9) mannitol-1-phosphatase, (10) unspecified hexitol transport, (11) sorbitol -6-phosphate dehydrogenase, (12) unspecified, (13) lactate dehydrogenase, PTS, phosphotransferase system; 6PG, 6-phosphaogluconate; EMP, embeden-Meyerhhof-parnas reaction 9 + 10 and 12 may occur via PTS.