Review Article
Challenges in Enzymatic Route of Mannitol Production
Table 3
Km values of MDH for different substrates (source from BRENDA database).
| Km value [mm] | Km value [mm] maximum | Substrate | Organism | Commentary | Literature |
| 1.8 | 6.5 | D-Arabinitol | Rhodobacter sphaeroides | — | [41] | 0.44 | — | D-Fructose | Pseudomonas fluorescens | in 50 mm glycine/NaOH buffer at pH 10 | [55] | 16.3 | 79.2 | D-Fructose | Rhodobacter sphaeroides | — | [41] | 50.97 | — | D-Fructose | Thermotoga maritima | at 80°C and pH 6.1; at 80°C, pH 6.1 | [46] | 71 | — | D-Fructose | Leuconostoc mesenteroides | pH 5.3 | [52] | 32 | — | D-Glucitol | Rhodobacter sphaeroides | — | [41] | 0.29 | 21.8 | D-Mannitol | Platymonas subcordiformis | — | [56] | 0.29 | 21.8 | D-Mannitol | Rhodobacter sphaeroides | — | [41] | 0.29 | 21.8 | D-Mannitol | Saccharomyces cerevisiae | — | [57] | 1.2 | — | D-Mannitol | Pseudomonas fluorescens | recombinant protein | [58] | 5.51 | — | D-Mannitol | Thermotoga maritima | at 80°C and pH 6.1; | [41] | 12 | — | D-Mannitol | Leuconostoc pseudomesenteroides | pH 8.6, 30°C | [59] | 13.23 | — | D-Mannitol | Thermotoga maritima | at 60°C and pH 6.1 | [41] | 32 | — | D-Mannitol | Leuconostoc mesenteroides | pH 8.6 | [52] | 78 | — | D-Mannitol | Lactobacillus sanfranciscensis | 25°C | [16] |
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