Changes in 𝜸-Tubulin Protein Distribution in Zebrafish (Danio rerio) Oocytes and the Early Cleavage-Stage Embryo

Figure 4

Summary of the alternating orientation of centrosomes and nuclei during zebrafish early embryogenesis using epifluorescence microscopy. Schematic cartoons of simplified nuclei (n; red dots) and curvilinear arc-shaped/ring (A/R) or punctate (p) centrosomes (green lines or dots, resp.) are depicted above each representative micrograph doubly labeled with DAPI (red) and GTU-88 (green). (a) First mitosis. (b) 2-cell stage with each cell in anaphase/telophase. (c) 4-cell stage with cells in prometaphase/metaphase. (d) 4-cell stage with each cell in early anaphase. (e) 4-cell stage with cells in telophase. (f) 8-cell stage, (g) 16-cell stage. (h) 32-cell stage.