Research Article

Production of Alkaline Protease by Solvent-Tolerant Alkaliphilic Bacillus circulans MTCC 7942 Isolated from Hydrocarbon Contaminated Habitat: Process Parameters Optimization

Table 1

Morphological, cultural, and biochemical characteristics of UB2 strain.

CharacteristicDescription

(A) Morphological
 ShapeRod
 Arrangement Single
 Gram stainGram positive
 Motility+
 Endospore+
 Capsule

(B) Cultural
 Growth temperature (°C)
  15
  25 +
  37 +
  40 +
  42 +
  45 +
  55 +
  65
 Growth pH
  5.0
  6.0
  7.0
  8.0+
  9.0+
  10.0++
  11.0+
 NaCl (% tolerance)
  2.5 +
  5.0
  7.0
  8.5
  10.5

(C) Biochemical
 Utilization of
  Malonate +
  Ribose +
  Xylose+
  L-Arabinose
  Glucosamine +
  Inositol
  Glucose+
  Fructose+
  Mannitol+
  D-Sorbitol+
  Maltose+
  Trehalose+
  Melibiose
  Sucrose+
  Cellobiose +
  Lactose
  Melezitose
  Raffinose
  Inulin
 Enzyme
  Amylase +
  Arginine dihydrolase +
  Catalase +
  Gelatinase+
  Lysine dehydrogenase
  Nitrate reductase
  Ornithine decarboxylase
  Oxidase+
  Urease