Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production

Table 4

CMCase production at different levels of optimization.

Process conditions	Level of scale	CMCase activity (U/mL)	Enhanced production (fold)
Without any optimization	Shake flask	0.07 ± 0.02	—
With only optimised medium	Shake flask	0.43 ± 0.04	6
With optimised medium + optimised physical parameters	Shake flask	0.57 ± 0.01	8
With optimised medium + optimised physical parameters	Bioreactor	0.75 ± 0.03	11

Values are mean ± SE (𝑛=3).