Gene Expression Analysis of the IPEC-J2 Cell Line: A Simple Model for the Inflammation-Sensitive Preterm Intestine

Table 1

Gene symbol, forward and reverse primer sequences, and amplicon length.

Gene symbol	Sequence (5′-3′)	Amplicon; length
ACTB	F: CTACGTCGCCCTGGACTTC	76
	R: GCAGCTCGTAGCTCTTCTCC
ALPI	F: TCCCAGACATACAACGTGGA	90
	R: GGTCTGGTAGTTGGCCTTGA
AOAH	F: GTAATGGCATTTGGGGTGTC	97
	R: TCTCCCAGCAAAATGATTCC
APOA1	F: GTTCTGGGACAACCTGGAAA	86
	R: GCTGCACCTTCTTCTTCACC
CCL2	F: GCAAGTGTCCTAAAGAAGCAGTG	103
	R: TCCAGGTGGCTTATGGAGTC
CCL3	F: CCAGGTCTTCTCTGCACCAC	90
	R: GCTACGAATTTGCGAGGAAG
CCL5	F: CTCCATGGCAGCAGTCGT	121
	R: AAGGCTTCCTCCATCCTAGC
CD14	F: GGGTTCCTGCTCAGATTCTG	164
	R: CCCACGACACATTACGGAGT
CD40	F: TGAGAGCCCTGGTGGTTATC	90
	R: GCTCCTTGGTCACCTTTCTG
CD163	F: CACATGTGCCAACAAAATAAGAC	130
	R: CACCACCTGAGCATCTTCAA
CD200	F: TCCCCAGGAAGTTTTGATTG	84
	R: CCATGGTTCTTGCTGAAGGT
CLDN3	F: ATCGGCAGCAGCATTATCAC	94
	R: ACACTTTGCACTGCATCTGG
CRP	F: GGTGGGAGACATTGGAGATG	85
	R: GAAGGTCCCACCAGCATAGA
CXCL10	F: CCCACATGTTGAGATCATTGC	141
	R: GCTTCTCTCTGTGTTCGAGGA
C3	F: ATCAAATCAGGCTCCGATGA	76
	R: GGGCTTCTCTGCATTTGATG
DEFB1	F: ACCTGTGCCAGGTCTACTAAAAA	109
	R: GGTGCCGATCTGTTTCATCT
DEFB4A	F: CAGGATTGAAGGGACCTGTT	99
	R: CTTCACTTGGCCTGTGTGTC
FGG	F: GAATTTTGGCTGGGAAATGA	86
	R: CAGTCCTCCAGCTGCACTCT
HP	F: ACAGATGCCACAGATGACAGC	105
	R: CGTGCGCAGTTTGTAGTAGG
HPRT1	F: ACACTGGCAAAACAATGCAA	71
	R: TGCAACCTTGACCATCTTTG
IL1B	F: CCAAAGAGGGACATGGAGAA	123
	R: GGGCTTTTGTTCTGCTTGAG
IL1RN	F: TGCCTGTCCTGTGTCAAGTC	90
	R: GTCCTGCTCGCTGTTCTTTC
IL6	F: TGGGTTCAATCAGGAGACCT	116
	R: CAGCCTCGACATTTCCCTTA
IL8	F: TTGCCAGAGAAATCACAGGA	78
	R: TGCATGGGACACTGGAAATA
IL10	F: CTGCCTCCCACTTTCTCTTG	95
	R: TCAAAGGGGCTCCCTAGTTT
IL18	F: CTGCTGAACCGGAAGACAAT	100
	R: TCCGATTCCAGGTCTTCATC
IFNG	F: CCATTCAAAGGAGCATGGAT	76
	R: TTCAGTTTCCCAGAGCTACCA
ITIH41	F: ATGACAGCAAGCGAACAGTG	85
	R: GGGGATCCCTCTTGGTAATC
ITIH42	F: AGGCCCTCACCATATCACAG	110
	R: GTTGCCATCCAGGACTGTTT
LBP	F: CCCAAGGTCAATGATAAGTTGG	83
	R: ATCTGGAGAACAGGGTCGTG
LCT	F: CACTCAAAGCTGTGCAGGAC	144
	R: GGATCCTTGGCAGAGAAGTG
MUC1	F: GGATTTCTGAATTGTTTTTGCAG	116
	R: ACTGTCTTGGAAGGCCAGAA
MUC2	F: GCACGTCTGCAACAAGGAC	125
	R: CAAAGCCCTCCAGGCAGT
NFKBIA	F: GAGGATGAGCTGCCCTATGAC	85
	R: CCATGGTCTTTTAGACACTTTCC
NFKB1	F: CTCGCACAAGGAGACATGAA	97
	R: GGGTAGCCCAGTTTTTGTCA
OCLN	F: CGGTGAGAAGATTGGCTGAT	100
	R: TTTCAAAAGGCCTGGATGAC
PAFAH1B1	F: GCAAACTGGCTACTGTGTGAAG	113
	R: GCACAGTCTGGTCATTGGAA
PTGS2	F: AGGCTGATACTGATAGGAGAAACG	100
	R: GCAGCTCTGGGTCAAACTTC
RPL13A	F: ATTGTGGCCAAGCAGGTACT	76
	R: AATTGCCAGAAATGTTGATGC
SAA	F: TAAAGTGATCAGCAATGCCAAA	96
	R: TCAACCCTTGAGTCCTCCAC
SFTPA1	F: CATGGGTGTCCTCAGTTTCC	86
	R: CATCAAAAGCGACTGACTGC
SLC5A1	F: CTGCAAGAGAGTCAATGAGGAG	99
	R: CCGGTTCCATAGGCAAACT
TF	F: CTCAACCTCAAAACTCCTGGAA	82
	R: CCGTCTCCATCAGGTGGTA
TGFB1	F: GCAAGGTCCTGGCTCTGTA	97
	R: TAGTACACGATGGGCAGTGG
TLR2	F: GTTTTACGGAAATTGTGAAACTG	136
	R: TCCACATTACCGAGGGATTT
TLR4	F: TTTCCACAAAAGTCGGAAGG	145
	R: CAACTTCTGCAGGACGATGA
TNF	F: CCCCCAGAAGGAAGAGTTTC	92
	R: CGGGCTTATCTGAGGTTTGA
TNFAIP3	F: CCCAGCTTTCTCTCATGGAC	113
	R: TTGGTTCTTCTGCCGTCTCT