Rapid Tests versus ELISA for Screening of HIV Infection: Our Experience from a Voluntary Counselling and Testing Facility of a Tertiary Care Centre in North India
Table 1
Characteristics of human immunodeficiency virus (HIV) tests as per kit literatures.
Test
Principle
Specimen volume required
Antigen employed
Time to test result
Specimen type
Sensitivity
Specificity
SD Bioline HIV-1/2 3.0
Lateral flow immunochromatography
10 L
gp 41 and p24 for HIV-1 and gp 36 for HIV-2
5–20 minutes
Serum, plasma
Not specified
Not specified
Pareekshak HIV-1/2 Triline card test
Lateral flow immunochromatography
10 L
gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2
20 minutes
Serum, plasma
100%
99.49%
Pareekshak HIV 1/2 rapid test kit (Trispot)
Immunoconcentration
2 drops
HIV 1 and HIV-2 r (recombinant)-proteins
Immediate
Serum, plasma
100%
100%
Microlisa-HIV
Indirect ELISA
10–20 L
gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2
>1.5 hours
Serum, plasma
100%
99.5%
HIV: human immunodeficiency virus; ELISA: enzyme linked immunosorbent assay.