Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography

Table 1

Chromatographic conditions of proposed analytical method.

Parameters	Optimum condition
Mobile phase A	5 mM ammonium dihydrogen orthophosphate (PH = 3.0)
Mobile phase B	acetonitrile (HPLC grade)
Column	Acquity BEH C18 (100 mm × 2.1 mm i.d., 1.7 𝜇 particle size)
Flow rate	0.3 mL/min
Isocratic elution at	55 : 45, v/v
Detection	215 nm UV
Diluent	Acetonitrile : water (1 : 1)
Injection volume	5 𝜇L