Influence of Albizia lebbeck Saponin and Its Fractions on In Vitro Gas Production Kinetics, Rumen Methanogenesis, and Rumen Fermentation Characteristics

Table 3

Effects of A.  lebbeck saponins fractions on IVDMD, PF, MBM, and methane production.

Parameters

Diets

D1; (60R : 40C)

D2; (50R : 50C)

D3; (40R : 60C)

SEM

Control

GSE

Sap A

Sap B

Control

GSE

Sap A

Sap B

Control

GSE

Sap A

Sap B

Diet

Treatment

D * T

IVDMD%

54.85±0.13

61.84±0.84

63.26±0.18

54.62±0.71

70.07±0.67

68.80±0.93

74.63±0.79

75.24±0.25

67.86±0.15

63.11±0.34

69.25±1.24

64.25±0.06

0.645

0.744

1.289

PF

3.41±0.03

3.91±0.08

4.16±0.05

3.34±0.02

3.40±0.03

2.62±0.06

3.04±0.07

3.24±0.06

3.38±0.19

3.10±0.07

3.52±0.04

3.17±0.06

0.054

0.062

0.107

MBM; (mg)

37.99±0.56

53.87±2.06

59.83±0.20

36.58±0.77

47.50±1.11

19.46±3.16

38.63±2.80

45.85±1.76

46.62±1.89

35.49±2.05

51.83±1.94

37.95±2.14

1.763

2.036

3.526

CH4; (mL/gDM)

54.85±2.17

43.74±2.26

29.10±1.67

34.66±0.91

57.94±1.96

76.59±1.18

51.25±0.74

50.97±0.61

61.00±0.36

45.37±0.90

32.31±2.22

31.11±0.75

2.875

3.319

5.750

CH4; (mM/gDM)

2.20±0.09

1.75±0.09

1.17±0.07

1.39±0.04

2.32±0.08

3.07±0.05

2.05±0.03

2.04±0.02

2.44±0.014

1.82±0.04

1.29±0.09

1.25±0.03

0.115

0.133

0.230

M/TDS

0.49±0.01

0.34±0.02

0.22±0.00

0.31±0.01

0.41±0.02

0.55±0.01

0.34±0.01

0.33±0.00

0.28±0.03

0.35±0.01

0.23±0.02

0.24±0.01

0.021

0.024

0.041

D₁: high fiber diet, D₂: medium fiber diet, D₃: low fiber diet, GSE: gross saponins extract, IVDMD: in  vitro dry matter digestibility, PF: partition factor, MBM: microbial biomass, CH₄: methane, M/TDS: methane (mL) per 200 mg true digestible substrate; SEM: standard error of means.