Influence of Albizia lebbeck Saponin and Its Fractions on In Vitro Gas Production Kinetics, Rumen Methanogenesis, and Rumen Fermentation Characteristics

Table 7

Real-time PCR quantification of changes in rumen microbial population on supplementation of A.  lebbeck (saponins fractions).

Microbial groups	Control diet (D2)	D2 + GSE	D2 + Sap A	D2 + Sap B	SEM	CD
MMB	1.00	0.318	0.484	1.581	0.091	0.321
Protozoa	1.00	0.940	0.649	0.985	0.070	0.247
mcrA	1.00	0.584	3.198	3.392	0.881	N.S.

MMB: methanomicrobiales; mcr A: total methanogens quantified using mcr A gene; in control diet population of all microbes considered as 1.00 then relative abundance is calculated with treatment.