Mouse Bone Marrow-Derived Endothelial Progenitor Cells Do Not Restore Radiation-Induced Microvascular Damage
Figure 2
EPC characteristics by EPC culture assay and immunohistochemistry. (a) Morphological features of confluent EPCs; EPCs after 14 days in culture demonstrating distinct flat, spread out, cobblestone morphology. (b) EPCs plated on Matrigel; EPCs originated from Eng+/+ mice (c) and Eng+/− mice show initial capillary tube formation after 6 hours in Matrigel. (d) DiI-acLDL uptake in red and (e) binding of UEA-1 in EPCs (green) were analyzed by fluorescent microscope. Nearly all adherent cells bound UEA and internalized Ac-DiI-LDL. Original magnification 63. (f) Immunohistochemical staining with the endothelial marker CD31 (in red). Staining was analyzed by fluorescent microscopy. Original magnification ×40.
