Novel Spectrophotometric Methods for the Determination of Selegiline Hydrochloride in Bulk and Its Pharmaceutical Preparation

Table 1

Spectral and statistical data for the determination of SEG.

Parameters	Method A	Method B
𝜆max (nm)	629	539
Beer’s law limits (μg/mL)	10.00–85.00	20.00–120.00
Molar absorptivity (L mol^−1 cm^−1)	0.98 × 10^4	0.94 × 10^4
Sandell’s sensitivity (μg cm^−2)	3.33 × 10^−2	1.98 × 10^−2
Limit of detection* (μg mL^−1)	0.8059	0.4347
Limit of quantification* (μg mL^−1)	2.4423	1.3173
Regression equation**	𝑌=𝑎+𝑏𝑋	𝑌=𝑎+𝑏𝑋
Slope (b)	0.0124	0.0046
Intercept (a)	0.1428	0.0047
Correlation coefficient (r)	0.9924	0.9987

∗Limit of detection calculated according to ICH guidelines.
** Y is the absorbance and X is the concentration in μg mL⁻¹.