Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography

Table 5

Determination of catechin and epicatechin in samples (; % relative standard deviations are given in parenthesis).

Sample		Catechin	Epicatechin
Cocoa powder^1		0.20% (0.72)	0.28% (0.55)
Chocolates	Serving size; (gm)	Catechin; per serving size	Epicatechin per serving size
Lindt Excellence, 90% cocoa^2	40	6.04 mg (0.27)	17.32 mg (0.17)
Lindt Excellence, 85% cocoa^2	40	4.80 mg (0.38)	22.76 mg (0.57)
Lindt Excellence, 70% cocoa^2	40	7.84 mg (0.32)	28.56 mg (0.24)
Ghirardelli Chocolate, 86% cacao^3	45	4.50 mg (0.51)	23.85 mg (0.28)
Ghirardelli Chocolate, 72% cacao^3	38	5.78 mg (0.34)	27.70 mg (0.33)
Ghirardelli Chocolate, 60% cacao^3	38	4.79 mg (0.32)	17.78 mg (0.23)
Godiva Chocolatier, 85% cacao^4	40	5.20 mg (0.79)	31.68 mg (0.08)
Safeway Select chocolate, 85% cacao^5	40	4.76 mg (0.83)	10.52 mg (1.04)
Safeway Select chocolate, 72% cacao^5	40	4.84 mg (0.86)	14.64 mg (0.69)
Green & Black’s chocolate, 50% cocoa^6	40	3.28 mg (0.36)	11.92 mg (0.14)
Bournville Cranberry chocolate, 50% cocoa^7	33	1.49 mg (1.25)	3.76 mg (0.18)
Bournville Rich Cocoa chocolate, 50% cocoa^7	33	1.09 mg (0.27)	3.60 mg (1.81)

¹Samples were supplied by M/S Natsol Laboratories Private Limited, Visakhapatnam, India.
²Lindt and Sprungli Inc., Stratham, NH 03885, USA.
³Ghirardelli Chocolate Company, San Leandro, CA 94578, USA.
⁴Godiva Chocolatier company, USA, product of Germany.
⁵Safeway Select Inc., P.O. Box  99, Pleasanton, CA 94566, USA, product of Switzerland.
⁶Green & Black’s, Parsippany, NJ 07054, USA, made in Italy.
⁷Cadbury India Limited, Mumbai, India.