Concentrated Hypoxia-Preconditioned Adipose Mesenchymal Stem Cell-Conditioned Medium Improves Wounds Healing in Full-Thickness Skin Defect Model

Figure 1

Characterization of adipose mesenchymal stem cells. The primary adipose mesenchymal stem cells were cultured in 7 days ((a)(1)) and exhibited a typical fibroblast-like morphology at passage 3 after 27 days ((a)(2)). For flow cytometry analysis, the results confirmed that the cells express CD29 (95.7%, (b)(1)), CD90 (98.8%, (b)(3)), and CD105 (99.1%, (b)(4)), but not CD34 (1.0%, (b)(2)). PE (0.6%, (b)(5)) and FITC (0.5%, (b)(6)) were performed as negative control. Oil Red O stain of lipid after differentiation for 28 days (before staining, (c)(1); after staining, (c)(2)), Alizarin Red staining of calcium after differentiation (before staining, (c)(3); after staining, (c)(4)), the Oil Red O stain of noninduced cells (before staining, (c)(5); after staining, (c)(6)), and the Alizarin Red stain of noninduced cells (before staining, (c)(7); after staining, (c)(8)). Scale bar = 100 μm.

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