Hyaluronan Is Not a Ligand but a Regulator of Toll-Like Receptor Signaling in Mesangial Cells: Role of Extracellular Matrix in Innate Immunity
Figure 6
Effect of heparin and hyaluronidase on the specific stimulation of TLR5. pMC/TLR4−/− (cells deficient of TLR4) were incubated with (a) TLR5 ligand with and without heparin and (b) different concentrations of a specific TLR5 ligand with and without hyaluronidase to demonstrate the effects of heparin and hyaluronidase on TLR stimulation. FLAG: flagellin; HYAL: hyaluronidase; HEP: heparin; data are means ± SD, ns: not significant, .
