Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas

<table>Comparison of IGKC : IGLC ratio between FC and RT-qPCR. The Pearson correlation coefficient is 0.65 (<svg height="11.375" id="M11" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>). The FC ratio refers to cell number, while the RT-qPCR ratio refers to transcript number. The areas where no monoclonality (MNP) could be proven are shown as dashed lines. Grey square, lymphadenitis; stars, diffuse large B cell lymphoma; triangles, chronic lymphocytic leukemia; dot, follicular lymphoma; circles, mantle cell lymphoma; <svg height="7.9499998" id="M12" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 8.7375002 7.9499998" width="8.7375002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M484 406q0 -21 -22 -38q-10 -7 -18 -1q-15 11 -30 11q-30 0 -77.5 -37t-74.5 -67l79 -168q11 -23 19.5 -34.5t13 -14t10.5 -2.5q9 0 30 17.5t40 42.5l17 -23q-31 -45 -57.5 -69.5t-40.5 -29.5t-31 -5q-18 0 -32 13.5t-35 55.5q-34 71 -69 160q-36 -32 -59 -56
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</svg>, IGKC; <svg height="12.4375" id="M13" style="vertical-align:-0.20064pt" version="1.1" viewbox="0 0 9.5124998 12.4375" width="9.5124998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, IGLC.</table>

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Figure 1

Figure 1: Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas 

Figure 1 | Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas 