Research Article

Development of SCAR Primers for PCR Assay to Detect Diplodia seriata

Table 2

SCAR primer sequences used in this study for the conventional PCR detection of Diplodia seriata.

TargetNameTypeSequence

Fragment 3.8DS3.8 S3Forward primer5′-ATCCTCATACTACGGCACGG-3′
DS3.8 R4Reverse primer5′-CCGTAGTCTCCCCTTTCCTC-3′
DS3.8 R6Reverse primer5′-AACGGTGACCCATTCCAC-3′