Research Article
Colicin E2 Expression in Lactobacillus brevis DT24, A Vaginal Probiotic Isolate, against Uropathogenic Escherichia coli
Table 1
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Description | Source |
| Lactobacillus brevis DT24 | Probiotic vaginal isolate | In this study | Escherichia coli NCTC 50133 | Contains pColE2-P9, produces colicin E2 | [50] | Escherichia coli DH5 | Transformation host | MTCC | Escherichia coli BL21 D3 | Expression Host | MTCC | Escherichia coli | Uropathogenic strain | MTCC |
| Plasmids | Description | Source |
| pSLP111.3 | Cmr, E. coli/LAB shuttle vector SlpA signal peptide | Prof. Jos Seeger Lactrys, The Netherlands | pColE2-P9 | Stpr, vector for colicin E2 | E. coli Genetic Stock Centre, Yale University | pSL-ColE2 | Cmr, SlpA, and ColE2 | In this study |
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