Colicin E2 Expression in Lactobacillus brevis DT24, A Vaginal Probiotic Isolate, against Uropathogenic Escherichia coli

Table 1

Bacterial strains and plasmids used in this study.

Strains and plasmids	Description	Source
Lactobacillus brevis DT24	Probiotic vaginal isolate	In this study
Escherichia coli NCTC 50133	Contains pColE2-P9, produces colicin E2	[50]
Escherichia coli DH5𝛼	Transformation host	MTCC
Escherichia coli BL21 D3	Expression Host	MTCC
Escherichia coli	Uropathogenic strain	MTCC
Plasmids	Description	Source
pSLP111.3	Cm^r, E. coli/LAB shuttle vector; SlpA signal peptide	Prof. Jos Seeger; Lactrys, The Netherlands
pColE2-P9	Stp^r, vector for colicin E2	E. coli Genetic Stock Centre, Yale University
pSL-ColE2	Cm^r, SlpA, and ColE2	In this study