Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

Table 1

Method validation parameters for quantification of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup by the proposed HPTLC method.

Validation parameters	Vasicine	Glycyrrhizin	Eugenol	Cineole
Correlation coefficient, = 3	0.99	0.99	0.99	0.97
Linearity range (ng/spot), = 3	300–900	1000–5000	400–1000	2000–10,000
Rf	0.53	0.44	0.75	0.77
Limit of detection (ng/spot), = 3	45	105	60	225
Limit of quantification (ng/spot), = 3	125	350	175	800
Instrumental precision (RSD), = 6	0.58	0.48	0.35	0.47
Method precision (RSD), = 6	0.72	0.59	0.42	0.58
Specificity	Specific	Specific	Specific	Specific
Robustness (RSD), = 3	0.92	0.94	0.85	0.92