Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

Table 2

Parameters used for ruggedness and robustness.

Sr. number	Parameter	Initial condition	Changed condition	Effect
1	Mobile phase	n-Hexane : ethyl acetate : glacial acetic acid (8.5 : 1 : 0.5)	n-Hexane : ethyl acetate : glacial acetic acid (8 : 1 : 1)	Very minute effect on the resolution, quantitative estimations, Rf, and peak area/height
2	Development distance	4 cm	3 cm	Very minute effect on the resolution, quantitative estimations, Rf, and peak area/height
3	Temperature	27 ± 3°C (winter)	35 ± 3°C (summer)	Affects greatly the resolution, quantitative estimations, Rf, and peak area/height
4	Tank saturation time	25 min	30 min	Very minute effect on the resolution, quantitative estimations, Rf, and peak area/height
5	Analyst	Analyst 1	Analyst 2	Very minute effect on the resolution, quantitative estimations, Rf, and peak area/height