Toxicity and Loss of Mitochondrial Membrane Potential Induced by Alkyl Gallates in Trypanosoma cruzi

Table 2

Synergism evaluation between hypothetical IC₅₀ values used for calculation of FIC index.

Compound	IC50; (gallates alone)	IC50; (gallates with benznidazole)	FIC index	Interaction type
Gallic acid (01)	294^a	17.4	2.017	I
Methyl gallate (02)	271^a	15.3	1.786	I
Ethyl gallate (03)	252^a	10.2	1.193	I
Propyl gallate (04)	235^a	8.90	1.043	I
Butyl gallate (05)	221^a	7.47	0.878	A
Pentyl gallate (06)	208^a	6.02	0.708	A
Hexyl gallate (07)	196^a	2.28	0.257	S
Heptyl gallate (08)	37.3	0.64	0.084	S
Octyl gallate (09)	6.49	0.62	0.165	S
Nonyl gallate (10)	0.86	0.44	0.560	A
Decyl gallate (11)	0.46	0.24	0.548	A
Undecyl gallate (12)	0.47	0.32	0.716	A
Dodecyl gallate (13)	0.72	0.51	0.765	A
Tetradecyl gallate (14)	6.44	0.58	0.155	S
Benznidazole^b	8.84	—	—	—

^aHypothetical IC₅₀ value used for calculating of FIC index.
^bPositive control.
I, A, and S mean indifferent interaction, antagonism, and synergism, respectively.