Studies on Shokyo, Kanzo, and Keihi in Kakkonto Medicine on Prostaglandin E₂ Production in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Figure 1

Effects of herbs on the production of PGE₂. HGFs were treated with combinations of LPS (0 and 10 ng/mL) and herb (0, 10, 30, and 100 μg/mL) for 24 h. Concentrations of PGE₂ were measured by ELISA, adjusted by cell number, and expressed as pg per 10,000 cells (mean ± SD, ). Open circles, treatment without LPS; closed circles, treatment with 10 ng/mL of LPS. , , and (without herb versus with herb). , , and (without LPS versus with LPS). values were calculated by pairwise comparisons and corrected with Holm’s method (10 null hypotheses).