Studies on Shokyo, Kanzo, and Keihi in Kakkonto Medicine on Prostaglandin E₂ Production in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Figure 4

Effects of herbs on COX and PGE synthase activities. HGFs were treated with LPS (10 ng/mL) and herb (100 μg/mL) for 8 h, washed, and then treated with (a) 10 μM arachidonic acid or (b) 10 nM PGH₂ for (a) 30 min or (b) 15 min. Concentrations of PGE₂ were measured by ELISA, adjusted by cell number, and expressed as pg per 10,000 cells (mean ± SD, ). values by Dunnett’s test are indicated. GR, kanzo; CC, keihi; ZR, shokyo; and NS-398, COX-2 inhibitor (20 μM) as a positive control.

(a)

(b)