Research Article

Studies on Shokyo, Kanzo, and Keihi in Kakkonto Medicine on Prostaglandin E2 Production in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Figure 6

Effects of herbs on LPS-induced ERK phosphorylation. HGFs were untreated (0 h), treated with LPS (10 ng/mL), or treated with both LPS and herb (100 μg/mL) for 30 min. Western blotting was performed using antiphosphorylated ERK or anti-ERK antibodies. pERK, phosphorylated ERK. Upper band indicates ERK1 (p44 MAPK) and lower band ERK2 (p42 MAPK). GR, kanzo; CC, keihi; and ZR, shokyo.