Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice
Figure 5
OPA inhalation stimulates B- and T-lymphocyte proliferation in the draining lymph nodes of mice. Mice were exposed to OPA or filtered air according to the schedule shown in Figure 1. Two days following the final exposure, the mandibular lymph nodes from the right side of the neck were removed and immediately processed into single-cell suspensions. Scatter properties were used to identify lymphocytes followed by fluorescent antibodies labeling to identify B lymphocytes, T lymphocytes (total, CD4+ and CD8+) using flow cytometry. Data are presented as fold change relative to the concurrent control. Statistical analysis was performed on the absolute cell counts for each lymphocyte population. Hatched bars represent mice that were exposed for 3 days followed by sacrifice on day 5 (sensitization exposure, Figure 1). Solid bars represent mice that were exposed on d1–d3, d16–d18 and sacrificed on d20 (sensitization/challenge exposure, Figure 1). Mean ± SEM (). *Significantly different from concurrent control group at . Absolute cell counts are presented in Table S2.
