Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice
Figure 6
OPA inhalation induces isotype switching in B lymphocytes to IgE. Mice were exposed to OPA or filtered air according to the schedule shown in Figure 1. Two days following the final exposure, the mandibular lymph nodes from the right side of the neck were removed and immediately processed into single-cell suspensions. Scatter properties were used to identify lymphocytes followed by fluorescent antibodies labeling to identify B lymphocytes using flow cytometry. Fluorescent anti-IgE antibodies were used to identify B-lymphocyte expressing IgE on their membrane. Open bars and open diamonds represent mice that were exposed OPA or filtered air, respectively, for 3 days followed by sacrifice on day 5 (sensitization exposure, Figure 1). Stippled bars and open triangles represent mice that were exposed OPA or filtered air, respectively, on d1–d3, d16–d18 and sacrificed on d20 (sensitization/challenge exposure, Figure 1). Mean ± SEM (). *Significantly different from concurrent control group at .
