Table 3: Type2 innatel cells (ILCs2) populations.

NameDate of discovery
and naming in the first publish
SpeciesTissue
distribution
Cytokines
produced
by cells
Stimulating
and
development
-effective
cytokines
Transcription
factors and
molecules
needed for
development
Surface phenotypeFunctions and pathologyReference

Natural helper
cells (NHCs)
(i) Fort et al. 2001 [40]
(ii) Hurst et al. 2002 [41]
(iii) Moro et al. 2010 [42]
Wild-type
mouse
FALT
Lung
*(i) IL-5, IL-13
  (ii) IL-4, IL-6, IL-2
IFN-
*(i) IL-25, IL-33
  (ii) IL-2/IL-25
  (iii) SCF/IL-7
  (iv) TSLP
*Id2, GATA-3,
Stat-6, c-Maf,
Jun-b, T-betlow,
ETS-1
Lin, c-kit (CD117), Sca1 (ly6a),
IL-7Rα (CD127), CD44,
IL-2Rα (CD25), ST2 (IL-33R),
CD45, CD69, CD90.2 (Thy1.2), Flt3
(i) Nematode expulsion
(ii) Induction of airway pathology following viral infection
(iii) Tissue repair following influenza virus infection
(iv) B1-B cell renewal
(v) IgA production by splenic B cells
(vi) Goblet cell hyperplasia
[4049]

Nuocytes(i) Neill et al. 2010 [50]IL-13-GFP
reporter mouse
Intestine
mLN
Spleen
*(i) IL-5, IL-13
 (ii) IL-4, IL-6, IL-10
 (iii) IL-2, GM-CSF
 (iv) CCL3 (MIP1 )
*(i) IL-25, IL-33
 (ii) IL-7
*Id2,
Notch-1, RoR- ,
GATA-3, c-Maf,
Stat-6, Jun-b
Lin, c-kit+/−, CD44, MHC II, Sca1,
IL-12RB, IL-7Rα +/low, IL-17RA,
ICOS (CD278), Klrg1, ST2+/−,
IL-10RB, IL-17RB (IL-25R), CD45,
CD90.2, IL-27R (wsx1),
ICAM-1, ICAM-2, CCR9, CXCR4,
CXCR6, B7 integrin
(i) Nematode expulsion
(ii) Increase in goblet cell mucin
(iii) Increase in T-cell response
[4345, 5052]

Innate helper2
(Ih2) cells
(i) Price et al. 2010 [53] IL-13-GFP
and
IL-4-GFP
reporter mouse
Broad, mostly in lung, spleen, mLN, and liver *(i) IL-5, IL-13
 (ii) IL-4
*(i) IL-25, IL-33
*Id2,
GATA-3,
Stat-6, aiolos
Lin, c-kit+/−/low, Sca1, CD45,
IL-2Rα, CD90.2, CD44, ST2, CD69
(i) Nematode expulsion
(ii) Increase in eosinophils
[43, 44, 5254]

Type2 innate
lymphoid cells
(ILCs2)
(i) Mjösberg et al. 2011 [55]Human(i) Fetal/adult gut and lung
(ii) Palatin tonsils
(iii) Nasal polyps
(iv) Adult
peripheral blood
*(i) IL-5, IL-13
*(i) IL-25/IL-2
*(ii) IL-33/IL-2
*Id2,
ETS-1, GATA-3
Lin, c-kit+/−, IL-7Rα, IL-2Rα,
ST2, IL-17RB, CD45,
CRTH2 (GPR44, CD294)
CD161 (NKR-P1A)
(i) Chronic rhinosinusitis
[47, 55]

Multipotent
progenitor
population 2
(MPPtype2)
Saenz et al. 2010 [56]IL-4-GFP
reporter mouse
(i) mLN
(ii) GALT especially in
Peyer’s patches
and cecal patch
*(i) IL-5, IL-13
 (mostly mRNA)
 (ii) IL-4
 (mostly mRNA)
*(i) IL-25
 (ii) SCF
 (iii) IL-3
*Id2,
c-Maf−/low,
GATA-3−/low
Stat-6−/low
Lin, c-kit, Sca1, CD45,
ST2−/low, IL-17RB, CD34−/low,
IL-7Rα −/low, CD62L−/low,
**MHC II
(i) Nematode expulsion
(ii) Increase in goblet cell mucin
(iii) Increase in T-cell response
[4345, 52, 56]

* The most important
** GFP cells expressed MHC II after cultivation with a combination of IL-3/SCF.
FALT: fat-associated lymphoid tissue, mLN: mesenteric lymph nodes, GFP: green fluorescent protein, GALT: gut-associated lymphatic tissues, Icos: inducible costimulatory molecule, CRTH2: chemoattractant receptor-homologous molecule expressed on Th2 lymphocytes, SCF: stem cell factor, GPR44: G protein-coupled receptor 44, ETS: E-twenty-six family, Flt3: Fms-related tyrosine kinase3.