Lung Dendritic Cell Developmental Programming, Environmental Stimuli, and Asthma in Early Periods of Life
Figure 3
The SDPCs harvested from a close-to-term fetal baboon differentiate into DCs when cultured in presence of GM-CSF, IL-4, and TNF-α. The lung SDPCs were harvested on OptiPrep density gradient as per the method published earlier [34]. (a) Photomicrograph showing cells with dendrites (*). (b) Flow cytometry data showing increase in DC-marker expression. Black line: isotype control antibody-stained cells, green line: 6 days, blue line: 11 days, red line: 17 days—cells stained with antibodies to particular marker. (c) Data in the table shows % cells (fluorescent intensity) gated in the marked region of histogram charts in (b) staining positive for the specific marker.
