Lithium Attenuates TGF--Induced Fibroblasts to Myofibroblasts Transition in Bronchial Fibroblasts Derived from Asthmatic Patients
Figure 7
P-Smad and β-catenin localization in non-asthmatic and asthmatic HBFs. HBFs from NA and AS were cultured in DMEM with 10% FCS for 24 hours, then in DMEM with 0.1% BSA for 24 hours and with the same medium supplemented with TGF- (5 ng/mL), LiCl (10 mM)s or TGF-/LiCl for 1 hour. Next, cells were immunostained for p-Smad2 (a) or β-catenin (b) and photographed with fluorescence microscopy. Arrows point to intensively stained nuclei. Bar = 50 μm.
