Figure 2: The presence of rNIF reduced IL-5 and IL-4 secretion into the BAL but not acute bronchial hyperreactivity. Forty-eight hours after the third challenge, bronchoalveolar lavage (BAL) was performed. The concentrations of IL-5 (; (a)) and IL-4 (ns; (b)) were measured by ELISA. Bronchial hyperreactivity (BHR) to methacholine nebulization was determined 24 h before sacrificing using whole-body plethysmography (c). BHR intensity was measured in Penh arbitrary units after indicated challenges and expressed as AUC values. The results represent means ± SD ( animals per group; * indicates ).