Figure 3: Role of rNIF in cell recruitment to the lungs and mucus hypersecretion. Lung sections of BALB/c mice sacrificed two days after the third challenge with either saline (NaCl), rNIF alone (NIF), OVA alone (OVA), and OVA with rNIF (OVA/NIF) are shown. The formalin-fixed tissue sections were stained with periodic acid Schiff reagent (PAS) to visualize mucus (a) and with hematoxylin/eosin to visualize cell recruitment (b), as shown by the arrows. Representative lung sections are shown at magnification ×20. V: blood vessel; B: bronchioles. Eosinophil recruitment (c) and goblet cell hyperplasia with mucus hypersecretion (d) were assessed semiquantitatively. Results represent means ± SD ( animals per group; * indicates ).