245909.fig.004a
(a)
245909.fig.004b
(b)
Figure 4: Recombinant NIF inhibits transendothelial migration of eosinophils but not neutrophils. Bilayer vascular constructs were activated with 1 ng/mL IL-4 for 16 h before transendothelial migration of eosinophils was determined in the presence of indicated concentrations of rNIF (a). In separate experiments bilayer vascular constructs were preincubated with 10 ng/mL TNF for 4 h. Thereafter, transendothelial migration of neutrophils was determined in the presence of indicated concentrations of rNIF (b). Graphs depict means of triplicate determinations ± SD representing typical results of a series of 3 independent experiments.