Table 1: Cumulative effects of blocking mAbs in the presence of rNIF on transendothelial migration of eosinophils across IL-4-activated HUVEC monolayers.
Antigen
Inhibition of eosinophil transendothelial migration (%)
mAb
Buffer
rNIF 10 nM
Mean
SD
Mean
SD
HLA I chain
W6/32
9.1
0.7
75.2
0.9
CD18
CLB54
71.3
1.9
100.4
0.4
VLA-4
HP2/1
41.7
2.2
85.7
0.9
CD11a/CD18
25.3
48.7
1.0
76.5
1.2
CD11b/CD18
60.1
71.3
0.9
67.8
1.2
CD11b A-domain MIDAS
107
65.2
1.0
66.1
1.0
Eosinophils were either incubated with the indicated mAbs alone or together with 10 nM rNIF. Data are given as mean of triplicate determinations ±SD.