Cyclin D1 in ASM Cells from Asthmatics Is Insensitive to Corticosteroid Inhibition
Figure 1
PDGF-BB upregulates cyclin D1 mRNA and protein expression in ASM from nonasthmatics and asthmatics. ASM cells from non-asthmatics and asthmatics were stimulated with 25 ng/mL of mitogen PDGF-BB. (a) demonstrates the temporal kinetics of cyclin D1 mRNA expression quantified by real-time RT-PCR (expressed as fold increase over 0 h). Statistical analysis was performed using one-way ANOVA, followed by Fisher’s post hoc multiple comparison test (* denotes a significant effect of PDGF-BB on cyclin D1 mRNA, compared to 0 h ()). (b) shows densitometric analysis of cyclin D1 protein expression at 24 h quantified by western blotting (expressed as fold increase over 0 h), using α-tubulin as the loading control. Statistical analysis was performed using the Student's unpaired -test (where * denotes a significant effect of PDGF-BB on cyclin D1 protein, compared to 0 h ()). Values are mean + SE.