Cyclin D1 in ASM Cells from Asthmatics Is Insensitive to Corticosteroid Inhibition
Figure 2
Dexamethasone inhibits cyclin D1 mRNA and protein expression in ASM cells from non-asthmatics. ASM cells from non-asthmatics were pretreated for 1 h with vehicle or dexamethasone and then stimulated with 25 ng/mL PDGF-BB. (a) demonstrates the effect of dexamethasone on the temporal kinetics of PDGF-BB-induced cyclin D1 mRNA expression (expressed as fold increase over 0 h). Statistical analysis was performed using two-way ANOVA, followed by Fisher’s post hoc multiple comparison test (where * denotes significant inhibition by dexamethasone ). (b) shows densitometric analysis of the effect of dexamethasone on cyclin D1 protein (expressed as fold increase over 0 h), using α-tubulin as the loading control. Statistical analysis was performed using the Student's unpaired -test (where * denotes a significant inhibition by dexamethasone ). Values are mean + SE.
