Role of the Arylhydrocarbon Receptor (AhR) in the Pathology of Asthma and COPD
Figure 4
(a) Effect of PPARγ agonist troglitazone on eosinophil chemotaxis stimulated with eotaxin. Purified eosinophils were preincubated with increasing concentrations of troglitazone for 1 h. Migration assays were performed using Boyden chambers. Chemotactic response to eotaxin alone was considered to be 100%, and reactions to lower concentrations are presented relative to eotaxin alone. Data are expressed as mean ± SD. Troglitazone inhibited the eotaxin-directed eosinophil chemotaxis in a dose-dependent manner . versus eotaxin alone. (b) Effect of troglitazone on eosinophil survival determined by staining with Annexin V-FITC and propidium iodine. Eosinophils were incubated with and without troglitazone in the presence of 1 ng/mL IL-5 for 48 h. Eosinophils were treated with Annexin V to stain early-phase apoptotic cells and with propidium iodine (PI) to stain the late-phase cells. The bar graph shows a dose-dependent effect of troglitazone on IL-5-induced eosinophil survival . Data are expressed as mean ± SD. versus without troglitazone.
