Figure 5: The impact of CD4+CD25+ Tregs in CLN of mice sublingually treated with antigen on Th2-cytokine production by effector T cells. CD4+CD25− effector T cells isolated from CLN of mice which developed allergic rhinitis without having sublingual treatment were incubated with CD4+CD25+ cells isolated from CLN of mice sublingually treated with either PBS or OVA in the presence of feeder cells and plate-bound anti-CD3 and soluble anti-CD28 antibodies for 96 hours. Th2-cytokine levels in culture supernatants were examined by cytokine ELISA. These data were obtained from two independent experiments containing three to five mice in each group. Significance was evaluated by an unpaired t-test. and .