Enhancement of Methacholine-Evoked Tracheal Contraction Induced by Bacterial Lipopolysaccharides Depends on Epithelium and Tumor Necrosis Factor
Figure 3
TNFα enhances methacholine response of isolated trachea. (a) Tracheal segments from C57BL/6 mice were incubated with TNFα (10 ng/mL) for 90 min (open circle) or 240 min (filled circle) in the presence of epithelium. Control tissues were obtained by incubating tracheas in the presence of epithelium with saline (filled triangle); (b) Tracheal segments from C57BL/6 mice were incubated with TNFα (10 ng/mL for 90 min) in the presence (filled circle) or in the absence (open circle) of epithelium. Control tissues were obtained by incubating tracheas with saline for 240 min (filled triangle) in the presence of epithelium. The contractile response to increasing concentrations of methacholine was recorded using a myograph. Data are expressed as mean ± SEM and are representative of 4 experiments (two-way ANOVA with Bonferroni's Multiple Comparison Test. *** as compared to control).
