Research Article

Comparison of CD63 Upregulation Induced by NSAIDs on Basophils and Monocytes in Patients with NSAID Hypersensitivity

Table 1

Comparison of NSAIDs and acetaminophen concentrations incubated with leukocytes to serum concentrations at usual therapeutic dosage and to 50% inhibitory concentrations (IC50) of cyclooxygenase-1 and -2.

DrugConcentrations
Tested*In serum**IC50***
mg/mLμMμMCOX-1COX-2

ASA0.01–0.1–131–310–3100 111 4.45 13.88
Diclofenac0.0013–0.013–0.13 4.2–42.2–422 6.1 0.26 0.01
Ketoprofen0.025–0.25–2.598.3–983–9830 9.4 0.11 0.88
Celecoxib0.005–0.05–0.513.1–131–1310 1.6 82 6.8
Acetaminophen0.01–0.1–166.1–661–6610 117 113.7 25.8

*Each NSAID and and acetaminophen (APAP) were tested at three ten-fold serial dilutions.
**Serum concentrations at usual therapeutic dosage.
***Concentration of drug that inhibited 50% of COX-1 in platelets or COX-2 in monocytes [1517].