Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
Figure 6
Induction of IL-1β and Nlrp3 protein expression in primary cell cultures of normal human bronchial epithelium by proinflammatory stimulus. Normal human bronchial epithelial (NHBE) cells were cultured without stimuli (control), or in presence of 50 ng/mL IFN-gamma (IFN), or 5 ug/mL E coli lipopolysaccharide (LPS), or their combination (IFN + LPS). Top and middle: fluorescence images of NHBE dual-labelled for intracellular Nlrp3 and IL-1β. Bottom: quantitation of fluorescence intensity by ImageJ software showing statistically significant increase (), when normalized to untreated controls, of both Nlrp3 and IL-1β in cells stimulated with LPS, or IFN + LPS combination. Error bars represent SEM. Expression of NRLP3 and IL1β was highly correlated within individual cells (, Spearman Rank correlation). Images are representative of 2 experiments with 30–50 cells analysed for each treatment.
