Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research

Figure 3

Immunofluorescent confocal imaging of differentiation markers. Localisation patterns of β-Tubulin IV, MUC5AC, and the Mouse IgG Isotype control at 28 days ALI were evaluated as described in the methods section. Single Z-slices are shown representing maximum intensity observed, with the corresponding Z-stack image below for β-Tubulin IV and MUC5AC. Scale bar represents 50 μm. Representative images are from three independent experiments. HBEC images were taken from experiments with low TEER (Figure 1).