Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research
Figure 4
Immunofluorescent confocal imaging of tight junction proteins. Localisation patterns of ZO-1, E-Cadherin, and the Rabbit IgG Isotype control at 28 days ALI were evaluated as described in the methods section. Images shown are single Z-stack slices representing maximum intensity observed with the corresponding Z-stack image below and are of matched fields using dual staining. The corresponding Mouse Isotype control for E-Cadherin can be seen in Figure 3. Scale bar represents 50 μm. Representative images are from three independent experiments. Images for HBEC Donor 1 were taken from an experiment reaching high TEER (>350 Ω·cm2), whilst Donor 2 images were taken from an experiment reaching low TEER (Figure 1).
